Article
Catalytic role of conserved HQGE motif in the CE6 carbohydrate esterase family.
FEBS letters - 2 Oct 2007
López-Cortés Nieves, Reyes-Duarte Dolores, Beloqui Ana, Polaina Julio, Ghazi Iraj, Golyshina Olga V, Ballesteros Antonio, Golyshin Peter N, Ferrer Manuel
Abstract excerpt
An acetylxylan esterase (R.44), belonging to the carbohydrate esterase family 6 (CE6), retrieved from bovine rumen metagenome was analyzed. Molecular modelling and site-directed mutagenesis indicated that the enzyme possesses a catalytic triad formed by Ser(14), His(231) and Glu(152). The catalytic Ser and His have been identified in highly conserved sequences GQSX and DXXH in the CE6 family, respectively, and...
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