Article
Effect of Cysteine Residue Substitution in the GCSAG Motif of the PMGL2 Esterase Active Site on the Enzyme Properties.
Biochemistry. Biokhimiia - 1 Jun 2020
Kryukova M V, Petrovskaya L E, Novototskaya-Vlasova K A, Kryukova E A, Yakimov S A, Nikolaeva A Y, Boyko K M, Dolgikh D A, Kirpichnikov M P
Abstract excerpt
The gene coding for PMGL2 esterase, which belongs to the family of mammalian hormone-sensitive lipases (HSLs), was discovered by screening a metagenomic DNA library from a permafrost soil. The active site of PMGL2 contains conserved GXSXG motif which includes Cys173 residue next to the catalytic Ser174. In order to clarify the functional role of the cysteine residue in the GCSAG motif, we constructed a number of...
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