Article
Involvement of prostaglandin-producing pathway in the cytotoxic action of tumor necrosis factor.
Cell structure and function - 1 Aug 1991
Hayakawa M, Oku N, Takagi T, Hori T, Shibamoto S, Yamanaka Y, Takeuchi K, Tsujimoto M, Ito F
Abstract excerpt
To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while no stimulation was observed at all in TNF-resistant cells tested. The cytotoxic action of TNF on...
Topics
- Animals
- Arachidonic Acid
- Binding Sites
- Cell Line
- Chromatography, High Pressure Liquid
- Dinoprostone
- Fibroblasts
- GTP-Binding Proteins
- Mice
- Mutation
- Pertussis Toxin