Article

A Novel Enzyme-Free Approach for Precursor microRNA Cloning and Expression

2023-12-11

Abstract excerpt

<title>Abstract</title> <p><bold>Background:</bold> Traditional cloning methods heavily depend on enzymatic digestion for generating sticky ends in DNA inserts, presenting challenges in efficiency and cost-effectiveness. MicroRNAs play crucial roles in biological processes and are integral to disease studies. Cloning microRNA precursors involves directional binding, necessitating meticulous techniques. Existing m...

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Literature Corpus work: 60e66ec8-377e-5827-b6d9-19c56f8f85d3
DOI: 10.21203/rs.3.rs-3502932/v1

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