Article
Vibrio cholerae fur mutations associated with loss of repressor activity: implications for the structural-functional relationships of fur.
Journal of bacteriology - 1 Aug 1994
Lam M S, Litwin C M, Carroll P A, Calderwood S B
Abstract excerpt
We used the Vibrio cholerae Fur protein as a model of iron-sensitive repressor proteins in gram-negative bacteria. Utilizing manganese mutagenesis, we isolated twelve independent mutations in V. cholerae fur that resulted in partial or complete loss of Fur repressor function. The mutant fur genes were recovered by PCR and sequenced; 11 of the 12 contained point mutations (two of which were identical), and one...
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