Article
Effect of sequence polymorphisms on performance of two real-time PCR assays for detection of herpes simplex virus.
Journal of clinical microbiology - 1 May 2005
Stevenson Jeffery, Hymas Weston, Hillyard David
Abstract excerpt
Herpes simplex virus (HSV) is the most common cause of acquired, sporadic encephalitis in the United States. PCR identification of HSV in spinal fluid has become the diagnostic gold standard due to its sensitivity and potential for speed, replacing other methods such as culture. We developed a real-time PCR assay to detect HSV, using a new type of hybridization probe, the Eclipse probe. In this study, we ran 323...
Topics
- Base Sequence
- Body Fluids
- DNA Primers
- Encephalitis, Viral
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Single Nucleotide
- Simplexvirus
- Temperature