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Article

Genetic design of conditional D-glutamate auxotrophy for Bacillus subtilis: use of a vector-borne poly-gamma-glutamate synthetic system.

Biochemical and biophysical research communications - 26 Oct 2007

Ashiuchi Makoto, Nishikawa Yoshito, Matsunaga Ken'ichiro, Yamamoto Masayoshi, Shimanouchi Kazuya, Misono Haruo

Abstract excerpt

Bacillus subtilis possesses two glutamate racemase isozymes, RacE and YrpC. For the first time, we succeeded in constructing glutamate racemase-gene disruptants of B. subtilis. Phenotypic analysis of their D-glutamate auxotrophy indicated that the RacE-type glutamate racemase is important for ensuring maximum growth rate but dispensable. The YrpC-type glutamate racemase probably operates as an anaplerotic enzyme...

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Identifiers

PMID: 17720142
DOI: 10.1016/j.bbrc.2007.08.019

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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