Article
Cloning and expression of the glutamate racemase gene of Bacillus pumilus.
Journal of biochemistry - 1 Jun 1997
Liu L, Yoshimura T, Endo K, Esaki N, Soda K
Abstract excerpt
A glutamate racemase gene (murI) was found in Bacillus pumilus cells and cloned into Escherichia coli WM335, a D-glutamate auxotroph, by means of a genetic complement method. MurI of B. pumilus encodes a 272-amino acid protein with an unusual initiation codon, TTG. The deduced amino acid sequence shows significant similarity with those of glutamate racemases from E. coli (ratio of identical residues, 28%),...
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