Article
Exploring novel function of yeast Ssa1/2p by quantitative profiling proteomics using NanoESI-LC-MS/MS.
Journal of proteome research - 1 Sept 2007
Matsumoto Rena, Nam Hyung Wook, Agrawal Ganesh Kumar, Kim Yu Sam, Iwahashi Hitoshi, Rakwal Randeep
Abstract excerpt
In the present study, we profiled proteins in ssa1/2 mutant and wild-type using one-dimensional gel electrophoresis coupled with liquid chromatography and mass spectrometry to reveal a total of 322 proteins. Sixty and 84 nonredundant proteins were detected in ssa1/2 and wild-type, respectively, whereas 178 were common. A quantitative profiling proteomic approach using a modified N-terminal isotope tagging method...
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