Article
Feasibility of detecting human immunodeficiency virus type 1 drug resistance in DNA extracted from whole blood or dried blood spots.
Journal of clinical microbiology - 1 Oct 2007
Steegen Kim, Luchters Stanley, Demecheleer Els, Dauwe Kenny, Mandaliya Kishor, Jaoko Walter, Plum Jean, Temmerman Marleen, Verhofstede Chris
Abstract excerpt
Due to high cost, availability of human immunodeficiency virus type 1 (HIV-1) drug resistance testing in resource-poor settings is still limited. We therefore evaluated the usefulness of viral DNA extracted from either whole blood or dried blood spots (DBS). Samples were collected from 50 patients receiving therapy and 10 therapy-naïve patients. Amplification and sequencing of RNA and DNA was performed using an...
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