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Article

Mutagenesis and gene identification in Dictyostelium by shotgun antisense.

Proceedings of the National Academy of Sciences of the United States of America - 14 May 1996

Spann T P, Brock D A, Lindsey D F, Wood S A, Gomer R H

Abstract excerpt

We have developed a mutagenesis technique that uses antisense cDNA to identify genes required for development in Dictyostelium discoideum. We transformed Dictyostelium cells with a cDNA library made from the mRNA of vegetative and developing cells. The cDNA was cloned in an antisense orientation immediately downstream of a vegetative promoter, so that in transformed cells the promoter will drive the synthesis of...

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Identifiers

PMID: 8643519
DOI: 10.1073/pnas.93.10.5003
PMCID: PMC39396

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