Article
Mutagenesis and gene identification in Dictyostelium by shotgun antisense.
Proceedings of the National Academy of Sciences of the United States of America - 14 May 1996
Spann T P, Brock D A, Lindsey D F, Wood S A, Gomer R H
Abstract excerpt
We have developed a mutagenesis technique that uses antisense cDNA to identify genes required for development in Dictyostelium discoideum. We transformed Dictyostelium cells with a cDNA library made from the mRNA of vegetative and developing cells. The cDNA was cloned in an antisense orientation...
Topics
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Antisense
- DNA, Complementary
- DNA, Fungal
- DNA, Protozoan
- Dictyostelium
- Gene Targeting
- Genes, Fungal
- Genes, Protozoan
- Genetic Vectors
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Phenotype
- Transformation, Genetic