Article
Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast.
Molecular & general genetics : MGG - 1 Mar 1991
Jeyaprakash A, Welch J W, Fogel S
Abstract excerpt
A 3.3 kb fragment of yeast genomic DNA was isolated by screening a genomic library constructed in the high copy number 2 micron plasmid YEp351 vector for clones capable of enhancing the degree of resistance of Saccharomyces cerevisiae strain MW3070-8B to cadmium. The insert contained two complete copies of the CUP1 gene open reading frame (183 bp), including the upstream promoter sequences (450 bp) with two...
Topics
- Amino Acid Sequence
- Blotting, Northern
- Cadmium
- Carrier Proteins
- Cloning, Molecular
- Copper
- DNA, Fungal
- Drug Resistance, Microbial
- Gene Amplification
- Genes, Fungal
- Metallothionein