Article
Gene inactivation mediated by Agrobacterium tumefaciens in the filamentous fungi Metarhizium anisopliae.
Applied microbiology and biotechnology - 1 Sept 2007
Staats Charley Christian, Junges Angela, Fitarelli Mariana, Furlaneto Marcia Cristina, Vainstein Marilene Henning, Schrank Augusto
Abstract excerpt
The list of fungal species with known complete genome and/or expressed sequence tag collections is extending rapidly during the last couple of years. Postgenomic gene function assignment is an obvious follow-up and depends on methodologies to test gene function in vivo. One of such methods is the generation of null mutants via homologous recombination at the wild-type loci by using inactivation cassettes. In this...
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