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Article

Large-scale insertional mutagenesis in Magnaporthe oryzae by Agrobacterium tumefaciens-mediated transformation.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Chen Xiao-Lin, Yang Jun, Peng You-Liang

Abstract excerpt

With genome sequences of more and more fungi become available, high-throughput systematic -mutagenesis is desirable for functional genomics studies. While a number of random insertional mutagenesis and targeted gene disruption approaches have been used in filamentous fungi, Agrobacterium tumefaciens-mediated Transformation (ATMT) remains one of the most effective methods for identifying genes required for...

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PMID: 21590424
DOI: 10.1007/978-1-61779-040-9_16

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