[Construction of CYP1A1-Wt and Asn461, Val462 gene eukaryotic expression vector and itstransfection in HLF cells].

Abstract excerpt

OBJECTIVE: To establish the cells model with high expression CYP1A1 and analyze the expression of the variable genotype. METHODS: Clone the target fragment (CYP1A1-Wt, Asn461, and -Val462) in T-Vectors into pBABE-neo, then transfer into the human embryonic lung fibroblast cells (HLF). The positive cells were selected by G418. The expression of CYP1A1 protein was measured by Western-Blot. At the same time, cells...

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