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Article

Phenotypical and functional characterization of a HepG2 cell clone stably overexpressing cytochrome P450 (CYP) 2C9.

BMC research notes - 30 Jun 2026

Kammerer Sarah, Herzog Natalie, Jenchen Thea, Stock Valentina, Hofer Rebecca, Ruzsanyi Veronika, Küpper Jan-Heiner

Abstract excerpt

OBJECTIVE: We aimed to generate a HepG2 cell clone stably overexpressing cytochrome P450 (CYP) 2C9 together with an empty vector (EV) control cell clone for pharmacological and toxicological studies of substances with CYP2C9-mediated biotransformation. RESULTS: A new HepG2 cell clone was generated by lentiviral transduction to functionally overexpress human CYP2C9. We found high CYP2C9 transcript and protein...

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Identifiers

PMID: 42380934
DOI: 10.1186/s13104-026-07935-y
PMCID: PMC13321531

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CYP2C9

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