Article
Mutations at the palmitoylation site of non-structural protein nsP1 of Semliki Forest virus attenuate virus replication and cause accumulation of compensatory mutations.
The Journal of general virology - 1 Jul 2007
Žusinaite Eva, Tints Kairit, Kiiver Kaja, Spuul Pirjo, Karo-Astover Liis, Merits Andres, Sarand Inga
Abstract excerpt
The replicase of Semliki Forest virus (SFV) consists of four non-structural proteins, designated nsP1-4, and is bound to cellular membranes via an amphipathic peptide and palmitoylated cysteine residues of nsP1. It was found that mutations preventing nsP1 palmitoylation also attenuated virus replication. The replacement of these cysteines by alanines, or their deletion, abolished virus viability, possibly due to...
Topics
- Animals
- Binding Sites
- COS Cells
- Chlorocebus aethiops
- Cricetinae
- Genes, Viral
- HeLa Cells
- Humans
- Mutation
- Palmitic Acids
- Replicon
- Semliki forest virus
- Subcellular Fractions