Article
RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors.
Cell - 1 Jun 2007
Gao Haixiao, Zhou Zhihong, Rawat Urmila, Huang Chenhui, Bouakaz Lamine, Wang Chernhoe, Cheng Zhihong, Liu Yuying, Zavialov Andrey, Gursky Richard, Sanyal Suparna, Ehrenberg Måns, Frank Joachim, Song Haiwei
Abstract excerpt
During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important...
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