Article
p53 alteration is a common event in the spontaneous immortalization of primary BALB/c murine embryo fibroblasts.
Genes & development - 1 Dec 1991
Harvey D M, Levine A J
Abstract excerpt
It has been shown previously that mutant p53 can act as an immortalizing gene when cotransfected into primary rat embryo fibroblasts along with a selectable marker. To determine whether a mutation at the p53 locus is a common event in the pathways leading to spontaneous cellular immortalization, 11 clonally derived BALB/c murine embryo fibroblast lines were established by passage on a 3T3 schedule and examined...
Topics
- Animals
- Base Sequence
- Cell Line
- Cell Separation
- Embryo, Mammalian
- Fibroblasts
- Half-Life
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mutation
- Selection, Genetic