Article
RNA mutagenesis yields highly diverse mRNA libraries for in vitro protein evolution.
BMC biotechnology - 11 Apr 2007
Kopsidas George, Carman Rachael K, Stutt Emma L, Raicevic Anna, Roberts Anthony S, Siomos Mary-Anne V, Dobric Nada, Pontes-Braz Luisa, Coia Greg
Abstract excerpt
BACKGROUND: In protein drug development, in vitro molecular optimization or protein maturation can be used to modify protein properties. One basic approach to protein maturation is the introduction of random DNA mutations into the target gene sequence to produce a library of variants that can be screened for the preferred protein properties. Unfortunately, the capability of this approach has been restricted by...
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