Article
In vitro improvement of a shark IgNAR antibody by Qbeta replicase mutation and ribosome display mimics in vivo affinity maturation.
Immunology letters - 15 Nov 2006
Kopsidas George, Roberts Anthony S, Coia Gregory, Streltsov Victor A, Nuttall Stewart D
Abstract excerpt
We have employed a novel mutagenesis system, which utilizes an error-prone RNA dependent RNA polymerase from Qbeta bacteriophage, to create a diverse library of single domain antibody fragments based on the shark IgNAR antibody isotype. Coupling of these randomly mutated mRNA templates directly to the translating ribosome allowed in vitro selection of affinity matured variants showing enhanced binding to target,...
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