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Article

Tailoring in vitro evolution for protein affinity or stability.

Proceedings of the National Academy of Sciences of the United States of America - 2 Jan 2001

Jermutus L, Honegger A, Schwesinger F, Hanes J, Plückthun A

Abstract excerpt

We describe a rapid and general technology working entirely in vitro to evolve either the affinity or the stability of ligand-binding proteins, depending on the chosen selection pressure. Tailored in vitro selection strategies based on ribosome display were combined with in vitro diversification by DNA shuffling to evolve either the off-rate or thermodynamic stability of single-chain Fv antibody fragments...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 11134506
DOI: 10.1073/pnas.98.1.75
PMCID: PMC14547

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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