Article
Biofunctional organic nanocrystals for quantitative detection of pathogen deoxyribonucleic acid.
Analytica chimica acta - 12 Feb 2007
Chan Cangel Pui-yee, Tzang Lawrence Chi-hung, Sin King-keung, Ji Sheng-lin, Cheung Kwan-yee, Tam Tze-kin, Yang Michael Meng-su, Renneberg Reinhard, Seydack Matthias
Abstract excerpt
Advances in nanotechnology have had significant impacts in the field of biodiagnostics. In this study, we describe the novel application of dissolvable, organic and biofunctional nanocrystals for the quantitative detection of a PCR product. Fluorescein diacetate (FDA), a fluorogenic precursor of fluorescein, was milled in a solution of a polymeric surfactant to create a stable, nanosized colloid with an interface...
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