Article
Identification of histone demethylases in Saccharomyces cerevisiae.
The Journal of biological chemistry - 11 May 2007
Tu Shengjiang, Bulloch Esther M M, Yang Lanhao, Ren Chen, Huang Wei-Chieh, Hsu Pang-Hung, Chen Chein-Hung, Liao Chung-Lin, Yu Hui-Ming, Lo Wan-Sheng, Freitas Michael A, Tsai Ming-Daw
Abstract excerpt
Based on the prediction that histone lysine demethylases may contain the JmjC domain, we examined the methylation patterns of five knock-out strains (ecm5Delta, gis1Delta, rph1Delta, jhd1Delta, and jhd2Delta (yjr119cDelta)) of Saccharomyces cerevisiae. Mass spectrometry (MS) analyses of histone H3 showed increased modifications in all mutants except ecm5Delta. High-resolution MS was used to unequivocally...
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