Article
Genotoxicity assessed by the comet and GPA assays following in vitro exposure of human lymphoblastoid cells (H9) or perinatal exposure of mother-child pairs to AZT or AZT-3TC.
Environmental and molecular mutagenesis - 1 Jan 2000
Escobar Patricia A, Olivero Ofelia A, Wade Nancy A, Abrams Elaine J, Nesel Carol J, Ness Roberta B, Day Richard D, Day Billy W, Meng Quanxin, O'Neill J Patrick, Walker Dale M, Poirier Miriam C, Walker Vernon E, Bigbee William L
Abstract excerpt
The genotoxicity of zidovudine (AZT) based treatments was investigated in human H9 lymphoblastoid cells in an in vitro study and in red blood cells (RBCs) from perinatally exposed HIV-1-infected mothers and their infants in an observational cohort study. Exposure of H9 cells for 24 hr to AZT produced dose-dependent increases in Comet assay tail moment (TM) when electrophoresed at pH 13.0, but not at pH 12.1 or pH...
Topics
- Anti-HIV Agents
- Cell Line
- Comet Assay
- Drug Combinations
- Erythrocytes
- Female
- Glycophorins
- Humans
- Infant
- Infant, Newborn