Article
Preclinical models for human pre-embryo biopsy and genetic diagnosis. II. Polymerase chain reaction amplification of deoxyribonucleic acid from single lymphoblasts and blastomeres with mutation detection.
Fertility and sterility - 1 Feb 1992
Morsy M, Takeuchi K, Kaufmann R, Veeck L, Hodgen G D, Beebe S J
Abstract excerpt
OBJECTIVE: To demonstrate the use of the polymerase chain reaction in the amplification of deoxyribonucleic acid (DNA) from single human lymphoblasts and mouse blastomeres. Amplified target genes for diagnosis of sickle cell anemia and Tay-Sachs are shown. Similarly, the sparce fur mouse model fo...
Topics
- Amino Acid Sequence
- Animals
- Biopsy
- Blastomeres
- DNA
- Embryo, Mammalian
- Genetic Diseases, Inborn
- Humans
- Lymphocytes
- Mice
- Molecular Sequence Data
- Mutation