Article
Human minisatellite alleles detectable only after PCR amplification.
Genomics - 1 Jan 1992
Armour J A, Crosier M, Jeffreys A J
Abstract excerpt
We present evidence that a proportion of alleles at two human minisatellite loci is undetected by standard Southern blot hybridization. In each case the missing allele(s) can be identified after PCR amplification and correspond to tandem arrays too short to detect by hybridization. At one locus, there is only one undetected allele (population frequency 0.3), which contains just three repeat units. At the second...
Topics
- Alleles
- Animals
- Base Sequence
- Blotting, Southern
- DNA Probes
- DNA, Satellite
- Female
- Humans
- Male
- Molecular Sequence Data
- Pedigree
- Phylogeny
- Polymerase Chain Reaction