Phenotypic conversion of TK-deficient cells following electroporation of functional TK enzyme.

doi:10.1016/0014-4827(92)90146-y

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Article

Phenotypic conversion of TK-deficient cells following electroporation of functional TK enzyme.

Experimental cell research - 1 Jan 1992

Dagher S F, Conrad S E, Werner E A, Patterson R J

Abstract excerpt

The ability to phenotypically rescue a mutant (Rat-3, thymidine kinase-deficient) cell line by electroporation of functional TK enzyme has been investigated. Extracts of electroporated cells showed a 35-fold increase in TK enzyme levels under conditions where greater than 90% of the cells remaine...

Topics

Animals
Autoradiography
Cell Line
Cytological Techniques
Electric Stimulation
Humans
Kinetics
Microscopy, Fluorescence
Phenotype
Rats
Recombinant Proteins
Thymidine
Thymidine Kinase

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