Article
Polarized and functional epithelia can form after the targeted inactivation of both mouse keratin 8 alleles.
The Journal of cell biology - 1 Dec 1991
Baribault H, Oshima R G
Abstract excerpt
We have tested the requirement of keratin intermediate filaments for the formation and function of a simple epithelium. We disrupted both alleles of the mouse keratin 8 (mK8) gene in embryonic stem cells, and subsequently analyzed the phenotype in developing embryoid bodies in suspension culture....
Topics
- Alleles
- Animals
- Base Sequence
- Cell Differentiation
- Cell Line
- DNA
- Epithelial Cells
- Epithelium
- Fluorescent Antibody Technique
- Gene Expression
- Keratins
- Mice
- Microscopy, Electron
- Molecular Sequence Data
- Mutation
- Precipitin Tests
- Restriction Mapping
- Stem Cells