Article
Experimental rugged fitness landscape in protein sequence space.
PloS one - 20 Dec 2006
Hayashi Yuuki, Aita Takuyo, Toyota Hitoshi, Husimi Yuzuru, Urabe Itaru, Yomo Tetsuya
Abstract excerpt
The fitness landscape in sequence space determines the process of biomolecular evolution. To plot the fitness landscape of protein function, we carried out in vitro molecular evolution beginning with a defective fd phage carrying a random polypeptide of 139 amino acids in place of the g3p minor coat protein D2 domain, which is essential for phage infection. After 20 cycles of random substitution at sites 12-130...
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