Article
Viewing protein fitness landscapes through a next-gen lens.
Genetics - 1 Oct 2014
Boucher Jeffrey I, Cote Pamela, Flynn Julia, Jiang Li, Laban Aneth, Mishra Parul, Roscoe Benjamin P, Bolon Daniel N A
Abstract excerpt
High-throughput sequencing has enabled many powerful approaches in biological research. Here, we review sequencing approaches to measure frequency changes within engineered mutational libraries subject to selection. These analyses can provide direct estimates of biochemical and fitness effects for all individual mutations across entire genes (and likely compact genomes in the near future) in genetically tractable...
Topics
- Animals
- Genetic Drift
- High-Throughput Nucleotide Sequencing
- Humans
- Models, Genetic
- Mutation
- Protein Conformation
- Protein Stability
- Proteins
- Selection, Genetic
- Sequence Analysis, DNA