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Article

Optimization of rice alpha-amylase production using temperature-sensitive mutants of Saccharomyces cerevisiae for the PHO regulatory system.

Biotechnology progress - 1 Jan 2000

Uchiyama K, Ohtani T, Morimoto M, Shioya S, Suga K, Harashima S, Oshima Y

Abstract excerpt

A typical example of rational system synthesis for bioproduction by the cultivation of microorganisms harboring a recombinant plasmid was studied. First, two temperature-controllable expression systems for a foreign gene in Saccharomyces cerevisiae were constructed. The promoter of the PHO84 gene...

Topics

Biotechnology
Genes, Regulator
Mathematical Computing
Mutation
Oryza
Plant Proteins
Saccharomyces cerevisiae
Sensitivity and Specificity
Temperature
alpha-Amylases

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Identifiers

PMID: 8546835
DOI: 10.1021/bp00035a003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8546835-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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