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Article

Chemical rescue of alpha3-galactosyltransferase. Implications in the mechanism of retaining glycosyltransferases.

Journal of the American Chemical Society - 20 Dec 2006

Monegal Ana, Planas Antoni

Abstract excerpt

The mechanism of retaining glycosyltransferases is still poorly understood and the subject of current debate. Both double displacement and front side single displacement (SNi) mechanisms have been proposed. A "chemical rescue methodology" is here applied to a retaining alpha3-galactosyltransferase. Azide as exogenous nucleophile rescues the activity of the inactive E317A mutant to give beta-d-galactosylazide....

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PMID: 17165744
DOI: 10.1021/ja0659931

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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