Article
Glycosyltransfer in mutants of putative catalytic residue Glu303 of the human ABO(H) A and B blood group glycosyltransferases GTA and GTB proceeds through a labile active site.
Glycobiology - 1 Apr 2017
Blackler Ryan J, Gagnon Susannah M L, Polakowski Robert, Rose Natisha L, Zheng Ruixiang B, Letts James A, Johal Asha R, Schuman Brock, Borisova Svetlana N, Palcic Monica M, Evans Stephen V
Abstract excerpt
The homologous glycosyltransferases α-1,3-N-acetylgalactosaminyltransferase (GTA) and α-1,3-galactosyltransferase (GTB) carry out the final synthetic step of the closely related human ABO(H) blood group A and B antigens. The catalytic mechanism of these model retaining enzymes remains under debate, where Glu303 has been suggested to act as a putative nucleophile in a double displacement mechanism, a local dipole...
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