Article
Demonstration of long-range interactions in a PDZ domain by NMR, kinetics, and protein engineering.
Structure (London, England : 1993) - 1 Dec 2006
Gianni Stefano, Walma Tine, Arcovito Alessandro, Calosci Nicoletta, Bellelli Andrea, Engström Ake, Travaglini-Allocatelli Carlo, Brunori Maurizio, Jemth Per, Vuister Geerten W
Abstract excerpt
Understanding the basis of communication within protein domains is a major challenge in structural biology. We present structural and dynamical evidence for allosteric effects in a PDZ domain, PDZ2 from the tyrosine phosphatase PTP-BL, upon binding to a target peptide. The NMR structures of its free and peptide-bound states differ in the orientation of helix alpha2 with respect to the remainder of the molecule,...
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