Article
A simple and reliable assay for detecting specific nucleotide sequences in plants using optical thin-film biosensor chips.
The Plant journal : for cell and molecular biology - 1 Jan 2007
Bai Su-Lan, Zhong Xiaobo, Ma Ligeng, Zheng Wenjie, Fan Liu-Min, Wei Ning, Deng Xing Wang
Abstract excerpt
Here we report the adaptation and optimization of an efficient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified (GM) crops and SNP markers in model plant genomes. Briefly, aldehyde-attached sequence-specific single-stranded oligonucleotide probes are arrayed and covalently attached to a...
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