Article
Construction of various mutants of xylose metabolizing enzymes for efficient conversion of biomass to ethanol.
Nucleic acids symposium series (2004) - 1 Jan 2006
Saleh Ahmed Abu, Watanabe Seiya, Annaluru Narayana, Kodaki Tsutomu, Makino Keisuke
Abstract excerpt
We applied protein engineering to construct an efficient biomass-ethanol conversion system using Saccharomyces cerevisiae. Intercellular redox imbalance caused by the different coenzyme specificity of xylose reductase (XR) and xylitol dehydrogenase (XDH) has been thought to be one of the main factors of xylitol excretion. Introduction of NADH-dependant XR generated in this study reduced the xylitol excretion...
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