Analysis and prediction of the physiological effects of altered coenzyme specificity in xylose reductase and xylitol dehydrogenase during xylose fermentation by Saccharomyces cerevisiae.

Abstract excerpt

An advanced strategy of Saccharomyces cerevisiae strain development for fermentation of xylose applies tailored enzymes in the process of metabolic engineering. The coenzyme specificities of the NADPH-preferring xylose reductase (XR) and the NAD⁺-dependent xylitol dehydrogenase (XDH) have been targeted in previous studies by protein design or evolution with the aim of improving the recycling of NADH or NADPH in...

Topics

• Aldehyde Reductase
• Coenzymes
• D-Xylulose Reductase
• Ethanol
• Fermentation
• Kinetics
• Metabolic Engineering
• Mutation
• NAD
• NADP
• Saccharomyces cerevisiae
• Saccharomyces cerevisiae Proteins
• Xylitol
• Xylose