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Article

A novel strictly NADPH-dependent Pichia stipitis xylose reductase constructed by site-directed mutagenesis.

Biochemical and biophysical research communications - 14 Jan 2011

Khattab Sadat Mohammad Rezq, Watanabe Seiya, Saimura Masayuki, Kodaki Tsutomu

Abstract excerpt

Xylose reductase (XR) and xylitol dehydrogenase (XDH) are the key enzymes for xylose fermentation and have been widely used for construction of a recombinant xylose fermenting yeast. The effective recycling of cofactors between XR and XDH has been thought to be important to achieve effective xylose fermentation. Efforts to alter the coenzyme specificity of XR and HDX by site-directed mutagenesis have been widely...

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Source

Identifiers

PMID: 21146502
DOI: 10.1016/j.bbrc.2010.12.028

Genes

XDH
HDX

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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