Article
Various mutations by using yeast gene for protein-engineering.
Nucleic acids symposium series (2004) - 1 Jan 2004
Watanabe Seiya, Kodaki Tsutomu, Makino Keisuke
Abstract excerpt
For construct an efficient-ethanol fermentation system from xylose by using yeast, several protein-engineering of xylitol dehydrogenase (XDH) were carried out. At first, we created a NADP+-dependent XDH by multiple site-directed mutagenesis. Furthermore, we succeeded to improve the thermostability by introduction of the structural zinc atom into XDH which does not possess a second zinc atom natively. Finally, the...
Topics
- Coenzymes
- D-Xylulose Reductase
- Enzyme Stability
- Kinetics
- Mutagenesis, Site-Directed
- Mutant Proteins
- Mutation
- NADP
- Pichia
- Protein Engineering
- Substrate Specificity
- Transition Temperature