Article
Colorimetric allele analysis based on the DNA-directed cooperative formation of luminous lanthanide complexes.
Nucleic acids symposium series (2004) - 1 Jan 2006
Kitamura Yusuke, Ihara Toshihiro, Tsujimura Yusuke, Osawa Yuka, Jyo Akinori
Abstract excerpt
We present the facile technique of colorimetric SNP analysis through DNA-templated cooperative complexation between a luminescent lanthanide ion (Ln(3+): Tb(3+) or Eu(3+)) and two ODN (oligodeoxyribonucleotide) conjugates carrying a metal chelator. Ethylenediaminetetraacetic acid (EDTA) and 1,10-phenanthrorine (phen) were covalently attached to ODNs to form the conjugate probes, capture and sensitizer probes,...
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