Article
Lattice stabilization and enhanced diffraction in human p38 alpha crystals by protein engineering.
Biochimica et biophysica acta - 14 Jan 2004
Patel Sangita B, Cameron Patricia M, Frantz-Wattley Betsy, O'Neill Edward, Becker Joseph W, Scapin Giovanna
Abstract excerpt
Mitogen-activated protein (MAP) kinase p38 alpha is activated in response to environmental stress and cytokines, and plays a significant role in inflammatory responses. For these reasons, it is an important target for the treatment of a wide range of inflammatory and autoimmune diseases. The crystals of p38 alpha that we obtained by published procedures were usually small, quite mosaic, and difficult to reproduce...
Topics
- Crystallization
- Drug Delivery Systems
- Drug Design
- Humans
- Mitogen-Activated Protein Kinase 14
- Mitogen-Activated Protein Kinases
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- X-Ray Diffraction