Article
Defining the roles of Asn-128, Glu-129 and Phe-130 in loop A of the 5-HT3 receptor.
Molecular membrane biology - 1 Jan 2000
Sullivan Nora L, Thompson Andrew J, Price Kerry L, Lummis Sarah C R
Abstract excerpt
The ligand binding pocket of Cys-loop receptors consists of a number of binding loops termed A-F. Here we examine the 5-HT3 receptor loop A residues Asn-128, Glu-129 and Phe-130 using modelling, mutagenesis, radioligand binding and functional studies on HEK 293 cells. Replacement of Asn-128 results in receptors that have wild type [3H]granisetron binding characteristics but large changes (ranging from a five-fold...
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