Article
Expression of foreign epitopes in P-fimbriae of Escherichia coli.
Molecular & general genetics : MGG - 1 Jul 1990
van Die I, van Oosterhout J, van Megen I, Bergmans H, Hoekstra W, Enger-Valk B, Barteling S, Mooi F
Abstract excerpt
Hypervariable regions (HRs) of the major subunit of F11 fimbriae were exploited for insertion of foreign epitopes. Two insertion vectors were created that contain a unique cloning site in HR1 or HR4 respectively. Several oligonucleotides, coding for antigenic determinants derived from different pathogens, were cloned in both insertion vectors. Hybrid fimbrial subunits were generally shown to be assembled in...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Viral
- Antigens, Bacterial
- Antigens, Viral
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- DNA, Viral
- Enzyme-Linked Immunosorbent Assay
- Epitopes