Article
Synthesis and expression in Escherichia coli of DNA encoding the murine lambda 1 chain of a monoclonal antibody specific for Salmonella serotype B O-antigen.
Protein engineering - 1 May 1990
Anand N N, Dubuc G, Mandal S, Phipps J, Gidney M A, Sinnott B, Young N M, MacKenzie C R, Bundle D R, Narang S A
Abstract excerpt
A 658 bp DNA sequence corresponding to the murine lambda 1 chain of a monoclonal antibody, Se155-4, specific for the Salmonella serotype B O-antigen, was designed using Escherichia coli preferred codons and chemically synthesized by ligation of synthetic fragments into a linearized plasmid follow...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Antigens, Bacterial
- Base Sequence
- Carbohydrate Sequence
- DNA, Bacterial
- DNA, Recombinant
- Escherichia coli
- Gene Expression
- Immunoglobulin lambda-Chains
- Mice
- Molecular Sequence Data