Article
Synthesis and expression in Escherichia coli of DNA encoding the murine lambda 1 chain of a monoclonal antibody specific for Salmonella serotype B O-antigen.
Protein engineering - 1 May 1990
Anand N N, Dubuc G, Mandal S, Phipps J, Gidney M A, Sinnott B, Young N M, MacKenzie C R, Bundle D R, Narang S A
Abstract excerpt
A 658 bp DNA sequence corresponding to the murine lambda 1 chain of a monoclonal antibody, Se155-4, specific for the Salmonella serotype B O-antigen, was designed using Escherichia coli preferred codons and chemically synthesized by ligation of synthetic fragments into a linearized plasmid followed by transformation into E. coli. A synthetic signal peptide (ompA) was fused to express the L chain as a free...
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