Article
Generation of lipooligosaccharide mutants of Haemophilus influenzae type b.
Journal of bacteriology - 1 Oct 1992
McLaughlin R, Spinola S M, Apicella M A
Abstract excerpt
We previously reported the analysis of recombinant plasmids from Haemophilus influenzae type b (Hib) that lead to modifications of Escherichia coli lipopolysaccharide (LPS) (Y. Abu Kwaik, R. E. McLaughlin, M. A. Apicella, and S. M. Spinola, Mol. Microbiol. 5:2475-2480, 1991). The modified LPS species are recognized by monoclonal antibodies (MAbs) 6E4 and 3F11. MAb 6E4 binds to a stable 2-keto-3-deoxyoctulosonic...
Topics
- Antigens, Bacterial
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- Haemophilus influenzae
- Lipopolysaccharides
- Mutagenesis, Insertional
- Mutation
- Plasmids
