Article
Linker insertion analysis of the FimH adhesin of type 1 fimbriae in an Escherichia coli fimH-null background.
FEMS microbiology letters - 1 Apr 1996
Schembri M A, Pallesen L, Connell H, Hasty D L, Klemm P
Abstract excerpt
The gene encoding the Escherichia coli FimH adhesin of type 1 fimbriae has been subjected to linker insertion mutagenesis. Amino acid changes were introduced at a number of positions spanning the entire sequence in order to probe the structure-function relationship of the FimH protein. The effect...
Topics
- Adhesins, Bacterial
- Adhesins, Escherichia coli
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA Primers
- Escherichia coli
- Fimbriae Proteins
- Fimbriae, Bacterial
- Genes, Bacterial
- Guinea Pigs
- Hemagglutination
- In Vitro Techniques
- Microscopy, Electron
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Phenotype