Article
Regulating expression of pyruvate kinase in Bacillus subtilis for control of growth rate and formation of acidic byproducts.
Biotechnology progress - 1 Jan 2000
Pan Zhiwei, Zhu Tao, Domagalski Nathan, Khan Saleem, Koepsel Richard R, Domach Michael M, Ataai Mohammad M
Abstract excerpt
Our prior work has shown that a pyk mutant of Bacillus subtilis exhibited diminished acidic byproduct accumulation, dramatically elevated phosphoenolpyruvate (PEP) pool, and reduced growth rate. To determine if a low acetate-producing but fast-growing strain of B. subtilis could be developed, we placed the expression of the pyk gene under the control of an inducible promoter. Enzyme measurements proved that PYK...
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