Article
Enhanced EGF mitogenic response is associated with enhanced tyrosine phosphorylation of specific cellular proteins in fibroblasts overexpressing c-src.
Oncogene - 1 Oct 1990
Wilson L K, Parsons S J
Abstract excerpt
In previous studies examining the role of pp60c-src in cellular proliferation, we demonstrated that overexpression of wild type (wt) but not mutated c-src in C3H10T1/2 murine embryo fibroblasts resulted in a 2 to 5 fold enhancement of DNA synthesis in response to epidermal growth factor (EGF). Using phosphotyrosine antiserum to probe Western immunoblots of whole cell lysates, we determined in the present study...
Topics
- Animals
- Cell Line
- DNA Replication
- Epidermal Growth Factor
- Fibroblasts
- Genes, src
- Immunoblotting
- Kinetics
- Mice
- Mutation
- Phosphorylation
- Phosphotyrosine