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Article

Positively charged C-terminal subdomains of EcoRV endonuclease: contributions to DNA binding, bending, and cleavage.

Biochemistry - 26 Sept 2006

Hiller David A, Perona John J

Abstract excerpt

The carboxy-terminal subdomains of the homodimeric EcoRV restriction endonuclease each bear a net charge of +4 and are positioned on the inner concave surface of the 50 degree DNA bend that is induced by the enzyme. A complete kinetic and structural analysis of a truncated EcoRV mutant lacking these domains was performed to assess the importance of this diffuse charge in facilitating DNA binding, bending, and...

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Identifiers

PMID: 16981705
DOI: 10.1021/bi0606400
PMCID: PMC2515858

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Source receipt: pubmed, retrieved 7 Sept 2026.

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